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Analytical Laboratories Applications

High Performance Liquid Chromatography (HPLC)

Using the optimum speciality gas and equipment when performing High Performance Liquid Chromatography (HPLC) will greatly improve the accuracy of your results.

HPLC is particularly useful for the separation of large molecular weight materials that have very low volatility and cannot be separated by gas chromatography. The main applications are within biotechnology and life sciences and the pharmaceutical industry.

HPLC uses a liquid mobile phase to separate the sample components. The components are dissolved in a solvent and then forced through the column at high pressure. The components then interact with the stationary phase and exit at different times in the same way as in gas chromatography. If excessive gas remains dissolved in the liquid mobile phase at column pressure, the gas can come out of the detector and cause unwanted sharp spikes. These can be eliminated by bubbling high purity Helium through the liquid in cases where the HPLC system does not have a built-in degasser. The Helium needs to have low levels of Hydrocarbons as these can dissolve in the solvent and produce baseline noise.

Our range of Experis® ultra high purity gases offers you the optimum gas for your HPLC requirements. Don’t forget that your choice of cylinder equipment also affects your analytical results. Our range includes high quality cylinder regulators, manifolds, valves and purge systems which will help to optimise the smooth operation and accuracy of your analysis.

The recommended gas and equipment for this application are listed below. Please note that our recommendation is based on common analytical requirements, so you may require a higher purity grade if analysing to lower concentrations or be able to use a lower purity grade if analysing to higher concentrations. If you require any additional advice on the right purity grade for your needs, please contact us.

Other Applications

Product NameDescription/BenefitsDownloads
Gases

Helium Premier/Premium Gas

When used for solvent degassing, Helium Premier/Premium gas with its low levels of Oxygen and Hydrocarbons is the ideal gas for this application.

When used for solvent degassing, Helium Premier/Premium gas with its low levels of Oxygen and Hydrocarbons is the ideal gas for this application.

Cylinder Regulators

Essential for reducing the gas pressure at the cylinder source, our range of high-quality cylinder regulators are available in single- or double-stage, a variety of materials such as brass, stainless steel and Monel® and various inlet and outlet pressure ranges.

Essential for reducing the gas pressure at the cylinder source, our range of high-quality cylinder regulators are available in single- or double-stage, a variety of materials such as brass, stainless steel and Monel® and various inlet and outlet pressure ranges.

Line Regulators

Essential for reducing the gas pressure in a pipeline, our range of high-quality line regulators is available in either brass or stainless steel, and various flow rate and outlet pressure ranges.

Essential for reducing the gas pressure in a pipeline, our range of high-quality line regulators is available in either brass or stainless steel, and various flow rate and outlet pressure ranges.

Manifolds

Essential for ensuring a batch or continuous supply, our range of high-quality manifolds can connect up to six cylinders, can provide manual or semi-automatic changeover and are available in brass or stainless steel.

Essential for ensuring a batch or continuous supply, our range of high-quality manifolds can connect up to six cylinders, can provide manual or semi-automatic changeover and are available in brass or stainless steel.

Pressure Gauges

Our high-quality, accurate pressure gauges are available in brass, stainless steel or Monel® for a wide variety of pressure ranges.

Our high-quality, accurate pressure gauges are available in brass, stainless steel or Monel® for a wide variety of pressure ranges.

Purge Systems

Purge systems are essential for eliminating impurities in and/or operator exposure to the gas when changing over cylinders. Our high-quality cross-purge, self-purge and T-purge systems are available in a variety of materials for the handling of pure, toxic, corrosive and flammable gases.

Purge systems are essential for eliminating impurities in and/or operator exposure to the gas when changing over cylinders. Our high-quality cross-purge, self-purge and T-purge systems are available in a variety of materials for the handling of pure, toxic, corrosive and flammable gases.

Valves

Our range of high-quality check valves and relief valves are available in brass or stainless steel and a variety of connection sizes for the connection of cylinders to equipment.

Our range of high-quality check valves and relief valves are available in brass or stainless steel and a variety of connection sizes for the connection of cylinders to equipment.

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